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One of the primary challenges faced by settlers in Plymouth, Massachusetts during their first winter was the severe shortage of food, which led to many settlers starving. This difficulty was compounded by harsh weather conditions and a lack of preparedness for the extreme cold and snow that characterized the region during winter months. The settlers arrived in the fall and were unable to grow crops in time for the winter, which, combined with their limited stored provisions, resulted in a dire food scarcity.

While harsh weather conditions were indeed a significant problem and affected their overall survival and ability to gather resources, the immediate consequence was indeed the lack of food and its devastating impact on the well-being of the settlers. This food shortage ultimately resulted in the deaths of many. Insufficient shelter was also a concern, as the settlers were not fully prepared for the severity of the winter. Conflict with Native Americans occurred but was not the primary challenge during that initial winter; rather, the struggle for survival due to starvation took precedence.